OE19 Cell Line human
96071721, human esophagus, Epithelial
Sinónimos:
JROECL19, JROECL19 Cells, OE-19 Cells, OEC19 Cells
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
41106514
Productos recomendados
Nombre del producto
OE19 Cell Line human, 96071721
origen biológico
human esophagus
modo de crecimiento
Adherent
cariotipo
Aneuploid
morfología
Epithelial
productos
Not specified
receptores
MHC class I
técnicas
cell culture | mammalian: suitable
enfermedades relevantes
cancer
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
Origen línea celular
Human Caucasian oesophageal carcinoma
Descripción línea celular
The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Aplicación
OE19 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study microRNA-330-5p as a modulator of neoadjuvant chemoradiotherapy sensitivity.
Study of oesophageal cancer
Perfil del ADN
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17
Medio de cultivo
RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Rutina de subcultivo
Split sub-confluent cultures (70-80%) 1:8, i.e., seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.
Otras notas
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.
Si necesita más asistencia, póngase en contacto con Atención al cliente
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico