CCRF S-180 II

90011901, mouse unknown/unspecified, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: CCRF S-180 II, 90011901
• biological source: mouse unknown/unspecified
• growth mode: Adherent
• karyotype: 2n = 40
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse CFW sarcoma 180

Cell Line Description

Established from an S-180 sarcoma maintained in adult CFW mice. The cells have been used extensively in chemotherapy screening tests.

Application

Chemotherapy screening tests

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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