HCA-7 Colony 3

9071502, human colon, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: HCA-7 Colony 3, 09071502
• biological source: human colon
• growth mode: Adherent
• karyotype: Near diploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human colorectal adenocarcinoma

Cell Line Description

Subpopulations isolated from the HCA-7 cell line.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 9,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16

Culture Medium

DMEM (High glucose Sigma D6546) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.05% trypsin/EDTA; 7.5% CO₂; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.