C9999
Citrate Buffer, pH 6.0, 10×, Antigen Retriever
for immunohistochemistry
Sinónimos:
1,2,3-Propanetricarboxylic acid, 2-hydroxy-, mixt. with sodium hydroxide (Na(OH)) and sodium 2-hydroxy-1,2,3-propanetricarboxylate
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About This Item
Código UNSPSC:
12352107
NACRES:
NA.47
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Aplicación
Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.
Nota de preparación
Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.
Almacenamiento y estabilidad
Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.
Otras notas
Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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