C4407
Human Collagen Type III
from human placenta, powder, suitable for cell culture
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About This Item
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Nombre del producto
Colágeno from human placenta, Bornstein and Traub Type III (Sigma Type X), powder
origen biológico
human placenta
Nivel de calidad
Ensayo
≥90% (GE)
Formulario
powder
técnicas
cell culture | mammalian: suitable
impurezas
HIV, hepatitis B and hepatitis C, none detected
solubilidad
aqueous acid: soluble
Nº de acceso UniProt
temp. de almacenamiento
2-8°C
Información sobre el gen
human ... COL3A1(1300)
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Aplicación
Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.
Componentes
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.
Precaución
This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years.
Nota de preparación
This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.
Nota de análisis
An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.
Otras notas
El colágeno se clasifica en una serie de distintos tipos desde el punto de vista estructural y genético. Nosotros utilizamos la nomenclatura propuesta por Bornstein y Traub. Tenga cuidado de no confundir las designaciones de tipo Sigma con los tipos de clasificación de colágeno reconocidos
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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