C4356

Anti-Caspase 9 antibody,Mouse monoclonal

clone CAS9, purified from hybridoma cell culture

• Número MDL: MFCD01864176
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: CAS9, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 37 kDa
• reactividad de especies: human
• concentración: ~2 mg/mL
• técnicas: indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using a purified recombinant human caspase 9 preparation
• isotipo: IgG1
• Nº de acceso UniProt: P55211
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CASP9(842)

Descripción

Descripción general

Monoclonal Anti-Caspase 9 (mouse IgG1 isotype) is derived from the CAS9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to amino acids of human caspase 9 protein, conjugated to keyhole limpet hemocyanin (KLH). Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspases are normally present in the cell as inactive procaspases. Caspase 9 (also known as Mch6 and ICE-LAP6) gene encodes a protein product of 37 kDa (reported also as 46 kDa), which undergoes a cleavage upon activation, leading to p18/p15/p9 subunits. It is a member of the CED-3 subfamily, bearing high similarity to caspase. High level expression of caspase 9 is found in the heart, testis and ovary.

Inmunógeno

synthetic peptide corresponding to amino acids 299-316 of human caspase 9 protein conjugated to KLH.

Aplicación

Anti-Caspase 9 antibody,Mouse monoclonal has been used in:

• western blot assay
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA).

Acciones bioquímicas o fisiológicas

Caspase 9 is a downstream protease, activated in caspase 3- and granzyme B-mediated apoptosis. Procaspase 9 is activated when a complex is formed with the cofactor apoptotic protease activating factor-1 (Apaf-1) through the caspase recruitment domain (CARD). Caspase 9 plays a critical role in the survival of neuronal progenitors within the embryonic forebrain and cortex. Its deficiency leads to defective brain development and embryonic lethality. Overexpression of caspase 9, but not of a mutant in which the catalytic Cys was replaced with an Ala, induces apoptosis in MCF7 cells.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable