A9935
Anti-Human IgM (μ-chain specific)−Agarose antibody produced in goat
affinity isolated antibody, PBS suspension
Sinónimos:
Anti Human IgM Antibody - Anti-Human IgM (μ-chain specific)-Agarose antibody produced in goat, Anti Human Igm Antibody
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
biological source
goat
Quality Level
conjugate
agarose conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
PBS suspension
technique(s)
Ouchterlony double diffusion: suitable
immunoelectrophoresis: suitable
capacity
2-3 mg/mL, resin binding capacity (human IgM)
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Goat polyclonal anti-Human IgM (μ-chain specific)−Agarose antibody specific for human IgM when tested against purified human IgA, IgG, IgM, and kappa and lambda light chains. No reactivity with mouse or rat IgG is observed by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Cyanogen bromide activated cross-linked beaded agarose is frequently conjugated to proteins such as immunoglobulins to facilitate their use in physicochemical processes such as precipitation, absorption and clarification.
Cyanogen bromide activated cross-linked beaded agarose is frequently conjugated to proteins such as immunoglobulins to facilitate their use in physicochemical processes such as precipitation, absorption and clarification.
Application
Anti-Human IgM (μ-chain specific)−Agarose antibody produced in goat has been used in affinity chromatography purification of human IgM.
Goat polyclonal anti-Human IgM (μ-chain specific)−Agarose antibody conjugate is used as an immuno-adsorbent to affinity purify antibodies, to remove species specific cross-reacting antibodies or to remove contaminating antibodies from an antiserum preparation. It has been used for IgM depletion from normal human serum from fasted patients.
Biochem/physiol Actions
IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid. IgM linked agarose resins have been tested for efficient conjugate binding.
Other Notes
Antibody adsorbed with mouse and rat IgG
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Rationally designed mimotope library for profiling of the human IgM repertoire
Pashov AD, et al.
bioRxiv, 308973-308973 (2018)
Anastas Pashov et al.
Frontiers in immunology, 10, 2796-2796 (2019-12-19)
Specific antibody reactivities are routinely used as biomarkers, but the antibody repertoire reactivity (igome) profiles are still neglected. Here, we propose rationally designed peptide arrays as efficient probes for these system level biomarkers. Most IgM antibodies are characterized by few
Solid?phase preparation of protein complexes.
Pengo P, et al.
Journal of Molecular Recognition, 23(6), 551-558 (2010)
Whitney Bullard et al.
Virulence, 3(1), 72-76 (2012-01-31)
The haptoglobin-hemoglobin receptor (HpHbR) of African trypanosomes plays a critical role in human innate immunity against these parasites. Localized to the flagellar pocket of the veterinary pathogen Trypanosoma brucei brucei this receptor binds Trypanosome Lytic Factor-1 (TLF-1), a subclass of
Jan Koopmans et al.
Cell transplantation, 13(6), 631-637 (2005-01-15)
Xenografting pig fetal ventral mesencephalic (pfVM) cells to repair the dopamine deficit in patients with Parkinson's disease is the focus of both experimental and clinical investigations. Although there have been marked advances in the experimental and even clinical application of
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico