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A5670

Sigma-Aldrich

Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-Pig Antibodies, Anti-Pig Antibodies - Anti-Pig IgG (whole molecule)-Peroxidase antibody produced in rabbit, Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit

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About This Item

MDL number:
MFCD00162752
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

pig

technique(s)

direct ELISA: 1:40,000
dot blot: 1:150,000 (chemiluminescent)

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. IgG is a major class of immunoglobulin. It has five subclasses of IgG- IgG1, IgG3, IgG2a and IgG2b. Affinity isolated antibody is obtained from rabbit anti-pig IgG antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to pig IgG. Anti-Pig IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.

Immunogen

purified pig IgG

Application

Anti-Pig IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense. Pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-pig IgG (whole molecule)-peroxidase antibody binds to pig IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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