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Merck

A4550

Sigma-Aldrich

Agar

Type A, suitable for plant cell culture

Sinónimos:

Agar goma, Agar-agar

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About This Item

Fórmula lineal:
(C12H18O9)n
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

type

Type A

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

gel strength

>650 g/cm2

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar (Type A) is extracted from agarocytes of the red alga Rhodophyceae. It is a polysaccharide complex, consisting of a neutral gelling fraction, agarose, and a non-gelling fraction, agaropectin. Type-A agar forms a thermoreversible gel, and is most suitable for plant cell culture.

Application

Type A agar has been used:
  • to perform metal(loid)-related phenotyping of the Arabidopsis thaliana metal-sensitive mutant strains
  • as a component of Murashige and Skoog (MS) media to culturing Arabidopsis thaliana seeds
  • to culture Cicer arietinum L. (Chickpea) seeds for Agrobacterium rhizogenes mediated root transformation

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Zhihang Feng et al.
Plant physiology, 184(1), 428-442 (2020-07-01)
Mg2+ is among the most abundant divalent cations in living cells. In plants, investigations on magnesium (Mg) homeostasis are restricted to the functional characterization of Mg2+ transporters. Here, we demonstrate that the splicing factors SUPPRESSORS OF MEC-8 AND UNC-52 1
Shimpei Uraguchi et al.
Frontiers in plant science, 11, 503-503 (2020-06-06)
For researchers in the plant metal field, the agar reagent used for the solid plate medium is a problematic factor because application of different agar types and even a different lot of the same agar type can mask the plant
Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using
Extraction and characterization of agar from Australian Pterocladia lucida
Chiovitti A, et al.,
Journal of Applied Phycology, 16(1), 41-48 (2004)
Pooja Rani Aggarwal et al.
Plant methods, 14, 55-55 (2018-07-11)
Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and

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