Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

View All Documentation

A3809

Sigma-Aldrich

Asparaginase from Escherichia coli

lyophilized powder, 100-300 units/mg protein (biuret)

Sinónimos:

L-Asparagine Amidohydrolase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(2)

About This Item

Número de CAS:
9015-68-3
Comisión internacional de enzimas:
3.5.1.1 (BRENDA, IUBMB)
EC Number:
232-765-3
MDL number:
MFCD00130565
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

100-300 units/mg protein (biuret)

composition

Protein, ≥60%

storage temp.

2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Asparaginase is a bacterial enzyme and also a chemotherapeutic drug.

Application

Asparaginase from Escherichia coli has been used:
  • to compare the cytotoxic effect of L-asparaginase purified from Streptomyces brollosae NEAE-115
  • as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues
  • to elicit amino acid deprivation

Biochem/physiol Actions

Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle. It is capable of causing pancreatitis in leukemia patients.
Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia . Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.
Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning .

Unit Definition

One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.

Physical form

Lyophilized powder containing sodium chloride

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H317,H361

Hazard Classifications

Repr. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Jie Xiong et al.
EBioMedicine, 72, 103614-103614 (2021-10-11)
Metabolic reprogramming plays an essential role on lymphoma progression. Dysregulation of glutamine metabolism is implicated in natural-killer T-cell lymphoma (NKTCL) and tumor cell response to asparaginase-based anti-metabolic treatment. To understand the metabolomic alterations and determine the potential therapeutic target of
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
Hitzel J, et al.
Nature Communications, 9(2292), 1-18 (2018)
Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling
Mukherjee A, et al.
Cellular and molecular gastroenterology and hepatology (2019)
Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115
El-Naggar NEA, et al.
BMC Pharmacology & Toxicology, 19(1), 1-15 (2018)
Changes in plasma angiotensin subtypes in Japanese eel acclimated to various salinities from deionized water to double-strength seawater
Wong MKS, et al.
General and Comparative Endocrinology, 178(2), 250-258 (2012)
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico