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92846

Sigma-Aldrich

5-Carboxyfluorescein N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Fórmula empírica (notación de Hill):
C25H15NO9
Número de CAS:
92557-80-7
Peso molecular:
473.39
Beilstein/REAXYS Number:
7865828
MDL number:
MFCD00077322
UNSPSC Code:
12352108
PubChem Substance ID:
57653496
NACRES:
NA.32

product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)

form

powder

fluorescence

λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI key

TWOKNYRPKCLMAR-UHFFFAOYSA-N

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers. Fluorescent probe for nucleotides; Reagent for fluorescent derivatization in capillary electrophoresis

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lau, S.K., et al.
Journal of Chromatography A, 809, 203-203 (1998)
G L Igloi
Analytical biochemistry, 233(1), 124-129 (1996-01-01)
A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of
L G Lee et al.
Nucleic acids research, 20(10), 2471-2483 (1992-05-25)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments
Bamaprasad Dutta et al.
Scientific reports, 7(1), 5765-5765 (2017-07-20)
Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation
Brandon L Blakely et al.
Nature methods, 11(12), 1229-1232 (2014-10-13)
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to

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