84120

Safranin O

for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)

• Sinónimos: Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Fórmula empírica (notación de Hill): C₂₀H₁₉ClN₄
• Número de CAS: 477-73-6
• Peso molecular: 350.84
• Número de índice de color: 50240
• Beilstein: 3924099
• Número CE: 207-518-8
• Número MDL: MFCD00011759
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 329769290
• NACRES: NA.47

Propiedades

• grado: for microscopy (Bact., Bot., Hist.); indicator (pH 0.3-1.0)
• Nivel de calidad: 200
• Formulario: powder or crystals
• técnicas: titration: suitable
• pH: 10 (20 °C, 10 g/L)
• solubilidad: methanol: 0.01 g/10 mL, red
• εmax: ≥1000 at 525-535 nm in 50% ethanol
• aplicaciones: diagnostic assay manufacturing; hematology; histology; microbiology
• temp. de almacenamiento: room temp
• cadena SMILES: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• Clave InChI: OARRHUQTFTUEOS-UHFFFAOYSA-N

Descripción

Descripción general

Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Aplicación

• Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores.
• It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei.
• It is a component of Benda′s polychrome stain.
• It is a component of various polychromes and lignin stains for botanical staining applications.
• It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections.

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Danger
• Frases de peligro: H318
• Consejos de prudencia: P280 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Dam. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves