83702

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

• Sinónimos: 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
• Fórmula lineal: C₂₁H₁₇ClN₂O₃
• Número de CAS: 62669-70-9
• Peso molecular: 380.82
• Beilstein: 6030951
• Número CE: 263-687-8
• Número MDL: MFCD00012664
• Código UNSPSC: 12352116
• ID de la sustancia en PubChem: 57652966
• NACRES: NA.32

Propiedades

• grado: for fluorescence
• Nivel de calidad: 100
• Línea del producto: BioReagent
• Ensayo: ≥85% (HPLC)
• Formulario: powder
• impurezas: ≤10% water
• solubilidad: ethanol: 20 mg/mL, clear, red
• idoneidad: in accordance for fluorescence
• cadena SMILES: Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
• InChI: 1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
• Clave InChI: MYFATKRONKHHQL-UHFFFAOYSA-N

Descripción

Descripción general

Rhodamine 123 is a fluorescent dye.

Aplicación

Rhodamine 123 has been used:

• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
• to label the cells for estimating the P-gp expression by flow cytometry
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
• the detection of mitochondrial membrane potential (ΔΨm)

Acciones bioquímicas o fisiológicas

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Otras notas

Supravital mitochondrial-specific dye

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)