83264

HEPES solution

1 M in H₂O, liquid

• Sinónimos: HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Número de CAS: 7365-45-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161700
• eCl@ss: 32129211
• ID de la sustancia en PubChem: 57652951
• NACRES: NA.25

Propiedades

• Nombre del producto: HEPES buffer solution, 1 M in H₂O
• Formulario: liquid
• Nivel de calidad: 100
• concentración: 1 M in H₂O; 238 g/L HEPES in H₂O
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5±0.5
• intervalo de pH útil: 6.8-8.2
• densidad: 1.07 g/mL at 20 °C
• aplicaciones: diagnostic assay manufacturing
• cadena SMILES: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descripción

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:

• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
• As a component of dissection buffer for culturing rat cortical neurons
• Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
• As a component of Danieu′s aquarium water used in agarose gel preparation

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)