81349

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

• Sinónimos: Poly(dI-dC) sodium salt
• Número de CAS: 118578-37-3
• Número MDL: MFCD00147947
• Código UNSPSC: 12352106
• NACRES: NA.52

Propiedades

• grado: for molecular biology
• Nivel de calidad: 100
• Formulario: powder
• calidad: lyophilized
• mol peso: M_r ~2.5 × 10⁶ (broad molecular weight distribution); M_r ~2500000
• envase: ampule of ≥10 units A₂₆₀
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Aplicación

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

Acciones bioquímicas o fisiológicas

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)