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Merck

50981

Sigma-Aldrich

Tiocianato de guanidina

BioReagent, for molecular biology, ≥99%

Sinónimos:

Rodanuro de guanidinio, Tiocianato de guanidinio

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About This Item

Fórmula lineal:
NH2C(=NH)NH2 · HSCN
Número de CAS:
Peso molecular:
118.16
Beilstein/REAXYS Number:
3563461
EC Number:
MDL number:
UNSPSC Code:
12352107
PubChem Substance ID:
NACRES:
NA.52

En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

synthetic

Quality Level

grade

for molecular biology

product line

BioReagent

assay

≥99%

form

powder

technique(s)

RNA extraction: suitable

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

color

colorless to white,

mp

115-122 °C
120-122 °C (lit.)

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Este artículo
G927750980RDD029
technique(s)

RNA extraction: suitable

technique(s)

RNA extraction: suitable

technique(s)

-

technique(s)

-

product line

BioReagent

product line

-

product line

BioUltra

product line

Redi-Dri

assay

≥99%

assay

≥99%

assay

≥99.0% (AT)

assay

≥99.0%

form

powder

form

powder or crystals

form

solid

form

-

biological source

synthetic

biological source

synthetic (organic)

biological source

-

biological source

-

Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis.[1] It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes.[2] It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.
Agente caotrópico y desnaturalizante fuerte; solubiliza las células.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation.[3] It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase).[4] GTC has been studied in the unfolding and refolding of fluorescent proteins (FP).[5] It can be used along with phenol and chloroform for extraction of RNA.[6]

Principle

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Visite la Librería de documentos

Standard methods for molecular research in Apis mellifera
Evans J D, et al.
Journal of Apicultural Research, 52, 1-54 (2013)
Nasir Ali et al.
BioMed research international, 2017, 9306564-9306564 (2017-08-09)
Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities
Isolation and Purification of RNA
Surzycki S
Basic Techniques in Molecular Biology, 119-144 (2000)
Extraction and Purification of Nucleic Acid using CBNP and PCIA Technique
Soni N
PharmaTutor, 4, 20-22 (2016)
Siun Chee Tan et al.
Journal of biomedicine & biotechnology, 2009, 574398-574398 (2009-12-17)
Extraction of DNA, RNA, and protein is the basic method used in molecular biology. These biomolecules can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes. In the past, the process of extraction and purification

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