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Merck
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Key Documents

481M-9

Sigma-Aldrich

PAX-7 (MRQ-69) Mouse Monoclonal Antibody

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

MRQ-69, monoclonal

description

For In Vitro Diagnostic Use in Select Regions

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (481M-94)
vial of 0.1 mL concentrate Research Use Only (481M-94-RUO)
vial of 0.5 mL concentrate (481M-95)
vial of 1.0 mL concentrate (481M-96)
vial of 1.0 mL concentrate Research Use Only (481M-96-RUO)
vial of 1.0 mL pre-dilute Research Use Only (481M-97-RUO)
vial of 1.0 mL pre-dilute ready-to-use (481M-97)
vial of 7.0 mL pre-dilute ready-to-use (481M-98)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (481M-98-RUO)

manufacturer/tradename

Cell Marque™

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:200 (concentrated)

isotype

IgG

control

Ewing’s sarcoma, rhabdomyosarcoma

shipped in

wet ice

storage temp.

2-8°C

visualization

nuclear

General description

The paired-box (PAX) family of proteins are key transcriptional regulators involved in early critical development.1 The PAX-7 transcription factor has important functions in mammalian myogenesis and early neural development, with a particularly crucial role in specification and self-renewal of skeletal muscle tissue.2,3 The expression of PAX-7 is highly restricted in normal adult tissues as demonstrated by nuclear immunoreactivity only being identified in rare, scattered satellite cells of the skeletal muscle and absent in both visceral smooth muscle and cardiac muscle as well as in a broad anatomical range of other non-neoplastic tissues. The assessment of soft tissue tumors and small round cell tumors is a persistent diagnostic challenge because of overlapping morphological features and insufficient molecular characterization in these groups of neoplasms. A high frequency of strong PAX-7 nuclear expression has been identified predominantly in rhabdomyosarcomas, preferentially in the embryonal subtype, and Ewing′s sarcoma, with reactivity being absent in related malignancies such as leiomyosarcoma, lymphoblastic lymphoma, neuroblastoma, carcinoid tumor, gastrointestinal stromal tumor, and small cell lung carcinoma. Immunohistochemical detection of PAX-7 protein can be used as a tool in distinguishing embryonal rhabdomyosarcoma and Ewing′s sarcoma from histologic mimics.4,5

Quality

United States - IVD
Canada - RUO
European Union - IVD
Japan - RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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Hugo C Olguin et al.
Developmental biology, 275(2), 375-388 (2004-10-27)
Satellite cells are myogenic precursors responsible for skeletal muscle regeneration. Satellite cells are absent in the Pax-7-/- mouse, suggesting that this transcription factor is crucial for satellite cell specification [Seale, P., Sabourin, L.A., Girgis-Gabardo, A., Mansouri, A., Gruss, P., Rudnicki
A Kawakami et al.
Mechanisms of development, 66(1-2), 119-130 (1997-08-01)
We describe here the isolation of chicken PAX6 and PAX7 as members of the PAX gene family expressed in late stages of chick nervous system development. By generating monoclonal antibodies against these PAX molecules, we analyzed their protein distributions in
Judith A Blake et al.
Development (Cambridge, England), 141(4), 737-751 (2014-02-06)
Pax genes encode a family of transcription factors that orchestrate complex processes of lineage determination in the developing embryo. Their key role is to specify and maintain progenitor cells through use of complex molecular mechanisms such as alternate RNA splice
Shunichi Toki et al.
Histopathology, 73(4), 645-652 (2018-06-20)
Ewing sarcoma is a small round cell tumour that affects bone and soft tissues. Although the detection of the specific fusion gene is a robust method of its diagnosis, immunohistochemistry may serve as a practical surrogate. Recent tissue microarray studies
Gregory W Charville et al.
The American journal of surgical pathology, 40(10), 1305-1315 (2016-08-16)
Rhabdomyosarcoma, the most common soft tissue malignancy of childhood, is a morphologically variable tumor defined by its phenotype of skeletal muscle differentiation. The diagnosis of rhabdomyosarcoma often relies in part on the identification of myogenic gene expression using immunohistochemical or

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