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22178

Sigma-Aldrich

Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

Sinónimos:

1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

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About This Item

Número de CAS:
9012-54-8
Comisión internacional de enzimas:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

100

form

powder

specific activity

~0.8 U/mg

color

off-white

storage temp.

2-8°C

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General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Application

Cellulase from Aspergillus niger has been used:
  • in the preparation of the standardized solution to determine absolute cellulase levels
  • in phosphate-citric acid buffer for activity assay
  • in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Unit Definition

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

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