10112303

Shmac 5 cell line

human prostate, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: Shmac 5 cell line, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human prostate, Epithelial
• origen biológico: human prostate
• envase: tube of 5 μg 10112303-DNA-5UG; pkg of vial of cells 10112303-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: See Lang SH, et.al (2006)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer

Descripción

Origen línea celular

Prostate cancer moderately well differentiated, early stage

Envase

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Perfil del ADN

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 12,14
D7S820: 7,12
THO1: 6,9.3
TPOX: 8,12
vWA: 15,17

Medio de cultivo

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable