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Merck

D0531

Sigma-Aldrich

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride

≥90% purity (HPLC), powder

Sinónimos:

DTAF

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About This Item

Fórmula empírica (notación de Hill):
C23H12Cl2N4O5 · HCl
Número de CAS:
Peso molecular:
531.73
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride, ≥90% (HPLC)

Quality Level

assay

≥90% (HPLC)

form

powder

color

yellow to orange

solubility

methanol: 20 mg/mL, clear to slightly hazy, yellow to orange

fluorescence

λex 495 nm; λem 516 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1

InChI

1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H

InChI key

CEKSHCAIHGNWBK-UHFFFAOYSA-N

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Application

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea). It has also been used to label bacteria (Vibrio spp.).
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.
A fluorescein derivative which reacts with amino groups and N-terminal amino acids of proteins at neutral pH.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures
Wang CS, et al.
Carbohydrate Polymers, 175, 484-492 (2017)
A gelation mechanism for gelatin/polysaccharide aqueous mixtures
Wang CS, et al.
Food Hydrocolloids, 79, 462-472 (2018)
Bruce L Granger
PloS one, 13(1), e0191194-e0191194 (2018-01-13)
Yeast wall protein 1 (Ywp1) is an abundant glycoprotein of the cell wall of the yeast form of Candida albicans, the most prevalent fungal pathogen of humans. Antibodies that bind to the polypeptide backbone of isolated Ywp1 show little binding
Assessment of fluorescent-labeled bacteria for evaluation of in vivo uptake of bacteria (Vibrio spp.) by crustacean larvae.
Soto-Rodriguez SA, et al.
Journal of Microbiological Methods, 52, 101-114 (2003)
Adrián Pérez-Ramos et al.
Frontiers in microbiology, 9, 1684-1684 (2018-08-10)
Lactic acid bacteria synthesize exopolysaccharides (EPS), which could benefit the host's health as immunomodulators. Furthermore, EPS could protect bacteria against gastrointestinal stress, favoring gut colonization, thus protecting the host against pathogenic infections. Pediococcus parvulus 2.6, produces a 2-substituted (1,3)-β-D-glucan and

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