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48722

Millipore

Gelatina from porcine skin

medium gel strength, suitable for microbiology

Sinónimos:

Gelatine, hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate

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About This Item

Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
41106212
NACRES:
NA.85

biological source

Porcine

Quality Level

sterility

non-sterile

form

powder

quality

medium gel strength

packaging

bottle of 100 g
bottle of 500 g

ign. residue

≤2%

loss

≤15% loss on drying

color

light yellow

pH

4.0-6.0 (25 °C, 67 mg/mL in H2O)

gel strength

165-195 g Bloom (67 mg/ml water)

solubility

H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow

anion traces

chloride (Cl-): ≤3000 mg/kg

cation traces

Ca: ≤2000 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤10 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤500 mg/kg
Mg: ≤500 mg/kg
Mn: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤10 mg/kg

application(s)

microbiology

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General description

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred to as type A. It is a hydrocolloid and is rich in glycine, proline, and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from porcine. Gelatin takes up a random coil structure after digestion from the triple helical collagen.

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc., in water. Type A gelatin is derived from acid-cured tissue, and Type B from lime-cured tissue.

The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 180.

Application

Gelatin has been used in many applications, such as in coating cell culture plates to improve attachment of cells, in PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology.

Components

La gelatina es una mezcla heterogénea de proteínas hidrosolubles de elevados pesos moleculares promedio, presentes en el colágeno. Las proteínas se extraen hirviendo en agua la piel, los tendones, los ligamentos, los huesos, etc.. La gelatina de tipo A procede de tejido curado con ácido. La B procede de tejido curado con cal.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. Manufactured by Gelita AG

Storage Class

11 - Combustible Solids

wgk_germany

nwg

ppe

Eyeshields, Gloves, type N95 (US)


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