10110434001
Roche
Xanthine Oxidase (XOD)
from cow milk
Sinónimos:
XOD
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About This Item
Productos recomendados
biological source
bovine milk
Quality Level
form
suspension
specific activity
~1 units/mg protein (At 25 °C with xanthine as the substrate.)
packaging
pkg of 1 mL (20 U)
manufacturer/tradename
Roche
optimum pH
8.5-9.0
shipped in
wet ice
storage temp.
2-8°C
General description
Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.
Xanthine:oxygen oxidoreductase
Application
Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.
Xanthine Oxidase has been used to study tyrosine nitration.
Biochem/physiol Actions
Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.
Quality
Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.
Note: Chromatographically purified.
Sequence
XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.
Unit Definition
One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E293nm = 12.2 mmol -1 x L x cm-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.
Physical form
Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8
Preparation Note
Activator: O2
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Los clientes también vieron
XANTHINE OXIDASE. VI. INFLUENCE OF PH ON SUBSTRATE SPECIFICITY.
The Journal of biological chemistry, 239, 1090-1095 (1964-04-01)
Synthesis and characterization of a novel organic nitrate NDHP: Role of xanthine oxidoreductase-mediated nitric oxide formation.
Redox Biology, 13, 163-169 (2017)
Methods of Enzymatic Analysis (2012)
The Journal of biological chemistry, 275(42), 32467-32474 (2000-07-25)
Peroxynitrite (ONOO(-)) is a potent nitrating and oxidizing agent that is formed by a rapid reaction of nitric oxide (NO) with superoxide anion (O(2)). It appears to be involved in the pathophysiology of many inflammatory and neurodegenerative diseases. It has
International Dairy Journal
Science, 16 (6), 546-554 (2006)
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