S8005H
CpGenome 5-hmC DNA Standard
CpGenome 5-hmC DNA St&ard contains a linear, double-str&ed DNA (897 bp) with 100% hydroxymethylated cytosine. This st&ard can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.84
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General description
CpGenome 5-hmC DNA Standard is a linear, double-stranded DNA (897 bp) containing 100% 5-hydroxymethylcytosine. This standard can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. This vial is a component of S8005 the CpGenome 5-mC and 5-hmC DNA Standard Set ( three linear, double-stranded DNAs (897 bp) with the same sequence, but containing either 100% 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines.
Materials Provided:
One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.
Validation:
Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.
CpGenome is a trademark of Serologicals Corporation.
Materials Provided:
One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.
Validation:
Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.
CpGenome is a trademark of Serologicals Corporation.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Physical form
Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Storage and Stability
Recommended Storage: Stable for 6 months at -20°C from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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