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PK1010

Sigma-Aldrich

PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr525/pTyr526)

Gene Information

human ... SYK(6850)

General description

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr525/526.
Recognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
This PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr525/526).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk

Application


ELISA (1:1000)
Immunoblotting (1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Analysis Note

Positive Control
293T cells transfected with wild-type Syk and CD8

Other Notes

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.
Tyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

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