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MCYTOMAG-70K

Millipore

Panel de microesferas magnéticas de citocinas/quimiocinas de ratón MILLIPLEX® - Ensayo múltiple de inmunología

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Sinónimos:

Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Assay

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Design And Price Your Kit

Nivel de calidad

200

reactividad de especies

mouse

fabricante / nombre comercial

Milliplex®

assay range

accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL

técnicas

multiplexing: suitable

método de detección

fluorometric (Luminex xMAP)

Condiciones de envío

wet ice

Descripción general

Para identificar citocinas específicas implicadas en cualquier respuesta inflamatoria o inmunitaria, podría ser necesario examinar paneles de citocinas, lo que a menudo requiere cierto nivel de automatización o un alto rendimiento. Las microesferas pueden facilitar el proceso de automatización y el cribado de alto rendimiento con funciones como el lavado a distancia. Las ventajas incluso fuera de la automatización son:

  • Opciones más flexibles de placa y lavadoras la de placa
  • Mejor rendimiento con muestras de suero o plasma turbias
  • Resultados de los análisis equivalentes a los obtenidos sin microesferas
  • El lavado automatizado evita muchos de los problemas asociados con el lavado con filtración al vacío


El panel de citocinas/quimiocinas de ratón MILLIPLEX® le permite concentrarse en el potencial terapéutico de las citocinas, así como en la modulación de la expresión de las citocinas. Junto con la plataforma Luminex® xMAP® en un formato de microesfera, obtendrá la ventaja de la velocidad y la sensibilidad ideales, lo que permite la detección cuantitativa múltiple de docenas de analitos simultáneamente y a su vez puede mejorar considerablemente la productividad.

Tipo de panel: Citocinas/quimiocinas

Aplicación

  • Analitos: G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α, VEGF, Eotaxina/CCL11

Características y beneficios

Diseñe su kit múltiple eligiendo los analitos disponibles en este panel.

Otras notas

Sensibilidad: Consulte el protocolo del kit para conocer las sensibilidades de cada una de las citocinas.

Información legal

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Órganos de actuación

Respiratory Tract

Código de clase de almacenamiento

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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M Emrah Şelli et al.
Journal of molecular and cellular cardiology, 107, 22-26 (2017-04-23)
Myocarditis, the principal cause of dilated cardiomyopathy and heart failure in young adults, is associated with autoimmunity to human cardiac α-myosin (hCAM) and the DR4 allele of human major histocompatibility II (MHCII). We developed an hCAM-induced myocarditis model in human
Lipopolysaccharide-induced brain activation of the indoleamine 2,3-dioxygenase and depressive-like behavior are impaired in a mouse model of metabolic syndrome.
Dinel, AL; Andre, C; Aubert, A; Ferreira, G; Laye, S; Castanon, N
Psychoneuroendocrinology null
Dectin immunoadhesins and pneumocystis pneumonia.
Ricks, DM; Chen, K; Zheng, M; Steele, C; Kolls, JK
Infection and Immunity null
Anna Martínez-Muriana et al.
Scientific reports, 6, 25663-25663 (2016-05-14)
Inflammation is a common neuropathological feature in several neurological disorders, including amyotrophic lateral sclerosis (ALS). We have studied the contribution of CSF1R signalling to inflammation in ALS, as a pathway previously reported to control the expansion and activation of microglial
Barbara Klein et al.
Frontiers in cellular neuroscience, 10, 169-169 (2016-07-23)
Allergies and their characteristic TH2-polarized inflammatory reactions affect a substantial part of the population. Since there is increasing evidence that the immune system modulates plasticity and function of the central nervous system (CNS), we investigated the effects of allergic lung
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Questions

1–9 of 9 Questions  

  1. Can the Cytokine Milliplex kits be used to measure cytokines in urine samples?

    1 answer

    1. Measurement of analytes in urine typically requires either a 24-hour urine collection or a second morning void collection. The analyte value is normalized against creatinine, meaning that the analyte is expressed as units/mg of creatinine.

      Helpful?

  2. How should data that are below the detection limit be analyzed in the case of a Cytokine Milliplex kit where there are quite a few samples below the detection limit, causing the distribution to be non-normal? Should these data be input as "0.00" or should the lower detection limit be used as the value?

    1 answer

    1. Most users will report the values as less than the manufacturer's limit of detection and then provide the sensitivity of each analyte in the Methods. Here are a couple of references as an example:

      - Impact of telmisartan on the inflammatory state in patients with coronary atherosclerosis--influence on IP-10, TNF-α and MCP-1. Klinghammer L, Urschel K, Cicha I, Lewczuk P, Raaz-Schrauder D, Achenbach S, Garlichs CD. Cytokine. 2013 May;62(2):290-6.

      - Plasma cytokine profiles in HIV-1 infected patients developing neuropathic symptoms shortly after commencing antiretroviral therapy: a case-control study. Van der Watt JJ, Wilkinson KA, Wilkinson RJ, Heckmann JM. BMC Infect Dis. 2014 Feb 10;14(1):71.

      Helpful?

  3. Can you confirm whether the detection antibodies of a multiplex kit are azide-free?

    1 answer

    1. The detection antibody cocktail is not azide-free.

      Helpful?

  4. Which chemicals can cause bleaching of the Luminex beads?

    1 answer

    1. The following solvents should not be used with Luminex microspheres as they will affect the classification dyes in the microspheres: Aromatic Hydrocarbons (Benzene, Toluene, Xylene, Ethylbenzene), Chlorinated aliphatic Hydrocarbons (Methylene chloride, Chloroform, Carbon tetrachloride), and Others (Pyridine, Dioxane, Dimethylformamide, Methyl ethyl ketone, Cyclohexanone, Tetrahydrofuran, N-butyl phthalate, Methyl phthalate, Ethyl phthalate, Tetrahydrofurfuryl alcohol, Ethyl acetate, Butyl acetate, 1-nitro-propane, Carbon disulfide, Tributyl phosphate, Cyclohexane, Methylcyclohexane, Ethylcyclohexane, Acetone, DMSO).

      High salt concentrations will also affect the classification of the microspheres on the Luminex 200 and FLEX 3D; as the salt concentration of the buffer increases, the microspheres will tend to spread out the bead map. High salt buffers (6X SSC, >0.2M NaCL) should be diluted or exchanged prior to analysis as they can interfere with microsphere classification.

      Helpful?

  5. Do you have guidelines available for preparing human and mouse adipose tissue for use in the Milliplex kits to measure cytokine levels?

    1 answer

    1. Our kits have not been validated for use with adipose tissue, although they can be used with various biological samples. Below are the guidelines for preparing adipose tissue:

      - Approximately 4 g of adipose tissue from each subject should be homogenized in 16 ml of ice-cold homogenization buffer containing 10% glycerol, 150 mM NaCl, 2 mM EDTA, 1 mM PMSF, 25 mM benzamidine, 10 µM leupeptin, 2.5 µmol/liter pepstatin A, and 50 U/ml aprotinin in 10 mM Tris-HCl (pH 7.0), using a Polytron (Brinkmann Instruments, Inc., Westbury NY) with four up/down strokes at Setting No. 3.
      - The crude homogenate should be centrifuged at 3,000 x g for 15 min, and the fat cake should be discarded. The homogenate should then be centrifuged again at 15,000 x g for 20 min at 4°C.
      - The supernatant should be stored in aliquots at -80°C.

      The details provided are sourced from the Journal of Clinical Endocrinology & Metabolism Vol. 86, No. 12 5973-5980, authored by Xiangdong Wu, Johan Hoffstedt, Wasim Deeb, Reetu Singh, Natalia Sedkova, Assaf Zilbering, Li Zhu, Pauline K. Park, Peter Arner, and Barry J. Goldstein.

      Helpful?

  6. What is the maximum number of samples that can be processed in one plate of the Milliplex Luminex Mouse Cytokine kit (MCYTOMAG-70K)?

    1 answer

    1. The Milliplex Luminex Mouse Cytokine kit (MCYTOMAG-70K) allows for processing 1 plate with 37 unknown samples and duplicates.

      Helpful?

  7. Is IL-18 for mouse available in the Milliplex kits?

    1 answer

    1. IL-18 is available through the Custom Assay program under the Cat# SPRCUS889. It includes concentrated IL-18 Standard, concentrated IL-18 Beads, and concentrated IL-18 Detection Antibody. This can be spiked into MCYTOMAG-70K or MTH17MAG-47K. The IL-18 bead region (63) does not overlap with any regions in these 2 panels.

      Helpful?

  8. Could you provide information about the antibody specificity of IL-12p40 and IL-12p70?

    1 answer

    1. The IL-12p40 antibody captures p40 on IL-12p40, IL-23, and is 30% cross-reactive to IL-12p70. For detection, it binds to p40 monomer, homodimer, and heterodimer, p35/p40. The IL-12p70 antibody, on the other hand, is specific to the p35 subunit and therefore only binds to IL-12p70. For detection, it binds to the p40 monomer, homodimer, and heterodimer.

      Helpful?

  9. Can you confirm or clarify whether the GRO antibody pair used in the Mouse Cytokine Panel 1 is a pan assay or specific to GRO1a?

    1 answer

    1. The GRO antibody in the MCYTOMAG-70K panel is specific to GROa/CXCL1, also known as KC or CINC-1.

      Helpful?

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