Saltar al contenido
Merck
Todas las fotos(2)

Documentos clave

MABS2314

Sigma-Aldrich

Anti-RHBDF2 Antibody, clone RHF2B 11H7

Sinónimos:

Rhomboid family member 2, Rhomboid veinlet-like protein 6, Rhomboid-related protein, iRhom2, inactive rhomboid protein 2

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

RHF2B 11H7, monoclonal

mol wt

observed mol wt ~95 kDa

purified by

using protein G

species reactivity

mouse, human

packaging

antibody small pack of 100

technique(s)

western blot: suitable

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 4

Este artículo
417821715445183
MRS Agar suitable for microbiology, NutriSelect® Basic

69964

MRS Agar

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

form

powder

form

powder

form

powder

form

powder

composition

agar, 12 g/L , D(+)-glucose, 20 g/L , manganous sulfate monhydrate, 0.05 g/L , sodium acetate, 5 g/L , universal peptone, 10 g/L , dipotassium hydrogen phosphate, 2 g/L , meat extract, 5 g/L , yeast extract, 5 g/L , magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L , triammonium citrate, 2 g/L

composition

agar, 15.0 g/L , ammonium citrate, 2.0 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20.0 g/L , dipotassium phosphate, 2.0 g/L , magnesium sulfate, 0.1 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3.0 g/L , proteose peptone (vegetable), 10.0 g/L , sodium acetate, 5.0 g/L , yeast extract, 5.0 g/L

composition

agar, 20 g/L , beef extract, 10 g/L , casein enzymic hydrolysate, 10 g/L , dextrose, 20 g/L , dipotassium phosphate, 2 g/L , polysorbate 80, 1 g/L , tomato juice, 2 g/L , yeast extract, 5 g/L

composition

agar, 10 g/L , disodium hydrogen phosphate monohydrate, 4 g/L , D(+)-glucose, 2 g/L , sodium azide, 0.4 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L , tryptose, 20 g/L , yeast extract, 5 g/L

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

agriculture
bioburden testing
environmental
food and beverages, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages, microbiology

application(s)

environmental
food and beverages, microbiology

application(s)

bioburden testing
clinical testing
environmental
food and beverages
water monitoring, microbiology

Specificity

Clone RHF2B 11H7 is a rat monoclonal antibody that detects Inactive rhomboid protein 2 (RHBDF2). It targets an epitope within 15 amino acids from the N-terminal half.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of mouse Inactive rhomboid protein 2 (RHBDF2).

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RHBDF2/iRhom2 in MCF-7 cell lysate.

Tested Applications

Western Blotting:

Lipopolysaccharide (LPS; 100 ng/mL; 24 h)-treated wild-type macrophage fractions from mouse bone marrow and RHBDF2 knockouts were probed with Cat. No. MABS2314, Anti-RHBDF2, clone RHF2B 11H7 (1:500 Dilution). Proteins were visualized using a Goat Anti-Rat IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RHBDF2 (~ 98 kDa). (Data courtesy: Katrin Moschke, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Munich.

Western Blotting Analysis: A representative lot detected RHBDF2 in Western Blotting applications (Weskamp, G., et al. (2020). J Biol Chem. 295(13):4350-4358).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Inactive rhomboid protein 2 (UniProt: Q80WQ6; also known as iRhom2, Rhomboid family member 2, Rhomboid veinlet-like protein 6, Rhomboid-related protein) is encoded by the Rhbdf2 (also known as Irhom2, Rhbdl6, Rhor) in murine species. RHBDF2/iRhom2, a multi-pass membrane protein with seven transmembrane domains, is predominantly localized in the endoplasmic reticulum (ER) membrane. It is reported to be crucial for the maturation and function of ADAM17 in myeloid cells. Hence, mice lacking this protein are protected from ADAM17- and TNF -dependent septic shock and inflammatory arthritis. ADAM17, a sheddase of the EGF receptor ligands and TNF , is shown to be involved in cell survival, proliferation, migration, and inflammation. However, it does not exhibit any protease activity on its own. RHBDF2 is involved in negative regulation of protein secretion and regulation of EGF receptor signaling pathway. By interacting with EGF, it may retain EGF in the endoplasmic reticulum and regulate its degradation through the ER-associated degradation (ERAD). (Ref.: Weskamp, G., et al. (2020). J. Biol. Chem. 295(13); 4350-4358; Li, X., et al. (2017). J. Cell Sci. 130(5); 868-878; Blaydon, DC., et al. (2012). Am. J. Hum. Genet. 90(2); 340-436; Zetti, M., et al. (2011). Cell. 145(1); 79-91).

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


  • Certificados de análisis (COA)

    Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

    ¿Ya tiene este producto?

    Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

    Visite la Librería de documentos

    Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

    Póngase en contacto con el Servicio técnico