HTS076RTA
Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells
Human
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.84
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product name
Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells, Human PAF GPCR frozen cells for Calcium Flux FLIPR Assays.
biological source
human
Quality Level
manufacturer/tradename
Ready-to-Assay
technique(s)
calcium flux assay: suitable
NCBI accession no.
detection method
fluorometric
shipped in
dry ice
General description
Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Platelet-activating factor (PAF) is a bioactive phospholipid that was originally characterized by its ability to induce platelet aggregation, but was subsequently shown to mediate angiogenesis, inflammation and neural development. The PAF receptor is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and cytokine and leukotriene release (Stafforini et al., 2003). Millipore’s cloned human PAF-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAF expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAF and its ligands.
Platelet-activating factor (PAF) is a bioactive phospholipid that was originally characterized by its ability to induce platelet aggregation, but was subsequently shown to mediate angiogenesis, inflammation and neural development. The PAF receptor is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and cytokine and leukotriene release (Stafforini et al., 2003). Millipore’s cloned human PAF-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAF expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAF and its ligands.
Cell Line Description
Host cells: Chem-1
Biochem/physiol Actions
Protein Target: PAF
Target Sub-Family: Platelet Activating Factor
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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