GF003AF
Fibroblast Growth Factor basic, human
Animal free, >95% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture
Sinónimos:
FGF-2, bFGF, basic-FGF, Heparin-Binding Growth Factor 2, Prostatropin
About This Item
Productos recomendados
product name
Fibroblast Growth Factor basic, human recombinant, animal-free, The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
biological source
human
Quality Level
assay
>95% (SDS-PAGE)
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
impurities
<0.1 EU/μg Endotoxin (of bFGF)
input
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
NCBI accession no.
UniProt accession no.
General description
Application
Physical form
Storage and Stability
General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.
For Human ES cell culture:
After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco′s PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).
Analysis Note
Legal Information
Disclaimer
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Fibroblast growth factors in cell culture and various growth factors for your research
Protocolos
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
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