ABE26
Anti-acetyl-c-Myc (Lys323) Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
species reactivity (predicted by homology)
rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys323)
Gene Information
chimpanzee ... Myc(464393)
human ... MYC(4609)
mouse ... Myc(17869)
rhesus monkey ... Myc(694626)
General description
c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell growth, differentiation, and proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling. c-Myc is itself acetylated on lysine 323 by GCN5, and on lysine 148 and 157 by p300/CBP. The significance of c-Myc acetylation is still under investigation; however, previous studies have suggested that HAT-dependent acetylation of c-Myc may regulate its turnover and stability. c-Myc may play a role in various cancers such as Burkitt’s lymphoma.
Specificity
Other homologies: Rat, Feline, Sheep, and Bovine (90% sequence homology).
This antibody recognizes c-Myc acetylated at Lys323.
Immunogen
Epitope: Acetylated Lys323
KLH-conjugated linear peptide corresponding to human c-Myc acetylated at Lys323.
Application
Peptide Inhibition Assay Analysis: 2 µg/mL from a representative lot blocked acetyl-c-Myc (Lys323) in HEK293T co-transfected with wild type c-Myc or mutant c-Myc (Lys323R) and P300.
Peptide Inhibition Assay Analysis: A representative lot was used by an independent laboratory in HEK293T cells co-transfected with mouse wildtype c-Myc and P300. (Image courtesy of Dr. Ernest Martinez, Department of Biochemistry, University of California at Riverside.)
Peptide Inhibition Assay Analysis: A representative lot was used by an independent laboratory in HEK293T cells co-transfected with mouse wildtype c-Myc and P300. (Image courtesy of Dr. Ernest Martinez, Department of Biochemistry, University of California at Riverside.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-acetyl-c-Myc (Lys323) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-c-Myc (Lys323) also known as HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC.
Quality
Evaluated by Western Blot in transfected HEK293T cell lysates.
Western Blot Analysis: 0.2 µg/mL of this antibody detected c-Myc on 10 µg of transfected HEK293T cell lysates.
Western Blot Analysis: 0.2 µg/mL of this antibody detected c-Myc on 10 µg of transfected HEK293T cell lysates.
Target description
~58 kDa observed. Uniprot describes two isoforms at 49 and 51 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Transfected HEK293T cell lysates
Transfected HEK293T cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Wesley W Wang et al.
Journal of the American Chemical Society, 143(40), 16700-16708 (2021-10-01)
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The clearance of apoptotic cells by macrophages (efferocytosis) prevents necrosis and inflammation and activates pro-resolving pathways, including continual efferocytosis. A key resolution process in vivo is efferocytosis-induced macrophage proliferation (EIMP), in which apoptotic cell-derived nucleotides trigger Myc-mediated proliferation of pro-resolving
Matthew Hurd et al.
Genes & development, 37(19-20), 865-882 (2023-10-19)
The MYC oncogenic transcription factor is acetylated by the p300 and GCN5 histone acetyltransferases. The significance of MYC acetylation and the functions of specific acetylated lysine (AcK) residues have remained unclear. Here, we show that the major p300-acetylated K148(149) and
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