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Key Documents

AB5354

Sigma-Aldrich

Anti-Internexin α Antibody

serum, Chemicon®

Sinónimos:

Anti-NEF5, Anti-NF-66, Anti-NF66, Anti-TXBP-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... INA(9118)

Specificity

alpha-internexin, C-terminal globular "tail" region. By western blot, AB5354 detects a major band at 66 kDa and a minor band at approximately 150 kDa corresponding to alpha internexin monomer and dimer, respectively. A minor band at approximately 50 kDa is occasionally detected and is believed to be a degradation product of alpha internexin. This antibody has been referred to in the literature as the R36 antibody (Evans, 2002).

Immunogen

Full length recombinant rat alpha-internexin fused to E. coli Trp E.

Application

Anti-Internexin Antibody, α is an antibody against Internexin for use in ELISA, WB, IC, IH.
Immunohistochemistry on formalin or paraformaldehyde fixed tissues.

Immunocytochemistry.

Western blot: using human or rat brain homogenates.

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Linkage

Replaces: 04-1032

Physical form

Serum liquid, containing no preservatives.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL CELLS: PC12, Ntera2.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Gene, 648, 42-53 (2018-01-18)
The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central
Thomas A Ray et al.
eLife, 7 (2018-04-04)
A common strategy by which developing neurons locate their synaptic partners is through projections to circuit-specific neuropil sublayers. Once established, sublayers serve as a substrate for selective synapse formation, but how sublayers arise during neurodevelopment remains unknown. Here, we identify
Frank Y Lee et al.
Journal of neuroscience research, 99(1), 67-89 (2020-05-22)
The function(s) of the Biogenesis of Lysosome-related Organelles Complex-1 (BLOC-1) during brain development is to date largely unknown. Here, we investigated how its absence alters the trajectory of postnatal brain development using as model the pallid mouse. Most of the
Tatsuo Suzuki et al.
Life science alliance, 4(7) (2021-05-20)
A purification protocol was developed to identify and analyze the component proteins of a postsynaptic density (PSD) lattice, a core structure of the PSD of excitatory synapses in the central nervous system. "Enriched"- and "lean"-type PSD lattices were purified by
Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons.
Yuan, A; Sasaki, T; Kumar, A; Peterhoff, CM; Rao, MV; Liem, RK; Julien, JP; Nixon, RA
The Journal of Neuroscience null

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