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Key Documents

36-003

Sigma-Aldrich

Anti-phospho-MYPT1 (Thr850) Antibody

Upstate®, from rabbit

Sinónimos:

Anti-GUBS, Anti-M130, Anti-MBS, Anti-MYPT1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken, rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr850)

Gene Information

human ... PPP1R12A(4659)

Specificity

Recombinant truncated chicken MYPT1 phosphorylated on Thr850.

Immunogen

Peptide corresponding to amino acid residues 845-855 of chicken MYPT1; this sequence is identical to amino acids 848-858 of human MYPT1, with the phosphorylated Thr at position 853.

Application

Detect phospho-MYPT1 (Thr850) using this Anti-phospho-MYPT1 (Thr850) Antibody validated for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Quality

Routinely evaluated by immunoblot on phophorylated MYPT1 substrate

Target description

80 kDa

Physical form

Format: Purified
Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
MYPT1 substrate phosphorylated by ROKa/ROCK-II

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Essential role for class II phosphoinositide 3-kinase alpha-isoform in Ca2+-induced, Rho- and Rho kinase-dependent regulation of myosin phosphatase and contraction in isolated vascular smooth muscle cells.
Yoshioka, K; Sugimoto, N; Takuwa, N; Takuwa, Y
Molecular Pharmacology null
Nandy C Lopez et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 310(11), R1053-R1063 (2016-02-26)
Exposure to high-altitude chronic hypoxia during pregnancy may cause pulmonary hypertension in neonates, as a result of vasoconstriction and vascular remodeling. We hypothesized that susceptibility to pulmonary hypertension, due to an augmented expression and activity of the RhoA/Rho-kinase (ROCK) pathway
Takeshi Takagi et al.
The Journal of thoracic and cardiovascular surgery, 142(2), e59-e65 (2011-03-15)
Radial arteries are increasingly used as conduits for coronary artery bypass grafts. However, vasospasm continues to be a major concern in radial artery grafts. Rho kinase plays a critical role in vascular contraction through phosphorylation of the regulatory subunit myosin
Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery.
Soo-Kyoung Choi,Duck-Sun Ahn,Young-Ho Lee
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Mechanically induced osteogenic differentiation--the role of RhoA, ROCKII and cytoskeletal dynamics.
Arnsdorf, EJ; Tummala, P; Kwon, RY; Jacobs, CR
Journal of Cell Science null

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