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Key Documents

345860

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10)

liquid, clone 2.2B10, Calbiochem®

Sinónimos:

Anti-GFAP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

2.2B10, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

bovine, rat, mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GCM1(8521)

General description

Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

Immunogen

Bovine Brain
bovine GFAP

Application

ELISA (0.1-0.5 µg/ml)

Immunoblotting (0.1-0.5 µg/ml)

Frozen Sections (10-50 µg/ml)

Immunoprecipitation (2-5 µg/ml)

Paraffin Sections (10-50 µg/ml, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human or rat brain tissues

Other Notes

Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
Tohyama, T., et al. 1993. Am. J. Pathol.142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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