219461
Cathepsin K, His•Tag®, Human, Recombinant, E. coli
Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.
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About This Item
Número de CAS:
UNSPSC Code:
12352202
NACRES:
NA.77
Productos recomendados
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥900 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag sequence.
Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His•Tag.
Note: 1 mU = 1 milliunit.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.
Physical form
In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Delaisse, J.M., et al. 2003. Microsc. Res. Tech.61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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