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Key Documents

16-200

Sigma-Aldrich

Anti-Histidine Tagged Antibody, clone 4D11, biotin conjugate

clone 4D11, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

conjugate

biotin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

4D11, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG2aκ

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HRG(3273)

Specificity

Recombinant proteins containing either an amino or carboxyl-terminal HHHHHHGS epitope tag, which is encoded by most His6 tag expression vectors. NOTE: The [GS] amino acids on the epitope tag are required for antibody recognition.

Immunogen

N-terminal His-tagged and truncated TRAP protein (amino acids 26-248) from P. falciparum expressed in E. coli. (catalog #05-531), clone 4D11.

Application

Anti-Histidine Tagged Antibody (anti His antibody), clone 4D11, biotin conjugate is an antibody against Histidine Tagged for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Routinely evaluated in western blot to detect recombinant N-terminal and C-terminal His-tagged proteins

Target description

The apparent molecular weight is related to the tagged protein.

Physical form

PBS, 0.05% sodium azide before the addition of glycerol to 30%
Protein G Purified

Storage and Stability

1 year at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificados de análisis (COA)

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Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal.
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In the present study, we investigated the prognostic roles of the O6-methylguanine-DNA methyltransferase (MGMT) gene methylation status, the protein profiles of MGMT, and the glioma stem cell (GSC) marker CD133 in malignant glioma resistance to radiotherapy. The proliferation of glioma
K J Robson et al.
The EMBO journal, 14(16), 3883-3894 (1995-08-15)
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First-line therapy for patients with glioblastoma multiforme includes treatment with radiation and temozolomide (TMZ), an oral DNA alkylating chemotherapy. Sensitivity of glioma cells to TMZ is dependent on the level of cellular O(6)-methylguanine-DNA methyltransferase (MGMT) repair activity. Several common coding-region

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