Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

1.52258

Supelco

Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Sinónimos:

Chromolith® WP 300 Protein A 25-4.6 HPLC column

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

product line

Chromolith®

feature

endcapped: no
endcapped: no

packaging

1 ea of

parameter

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

column L × I.D.

2.5 cm × 4.6 mm

surface area

120 m2/g

matrix

monolithic silica
monolithic silica

matrix active group

Protein A bonding phase
Protein A bonding phase

pore size

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5

operating pH

1.5-7.5

separation technique

affinity

storage temp.

2-8°C
2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Artículos

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Contenido relacionado

HPLC de moléculas grandes

La cromatografía de líquidos de alta resolución (HPLC) puede utilizarse para separar e identificar diferentes biomoléculas grandes, como proteínas y péptidos, en una muestra. Se basa en el bombeo de una muestra con un disolvente (fase móvil) a través de una columna rellena con material absorbente (fase estacionaria) a una presión elevada.

HPLC de moléculas grandes

La cromatografía de líquidos de alta resolución (HPLC) puede utilizarse para separar e identificar diferentes biomoléculas grandes, como proteínas y péptidos, en una muestra. Se basa en el bombeo de una muestra con un disolvente (fase móvil) a través de una columna rellena con material absorbente (fase estacionaria) a una presión elevada.

Large Molecule HPLC

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Large Molecule HPLC

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico