1.09333

LiChrosorb^® RP-18 (5 µm) HPLC Columns

HPLC sorbent

• Sinónimos: TM=["LiChrosorb"] C18 sorbent for HPLC
• UNSPSC Code: 41115709
• NACRES: SB.52

Propiedades

• product name: LiChrosorb^® RP-18 (5 µm),
• material: stainless steel column
• agency: suitable for USP L1
• product line: LiChrosorb^®
• feature: endcapped: no
• parameter: 60 °C max. temp.
• technique(s): HPLC: suitable; mass spectrometry (MS): suitable (Low)
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 10 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• bulk density: 200‑400 kg/m³
• separation technique: reversed phase
• storage temp.: no temp limit

Descripción

General description

LiChrosorb^® RP-18 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.

Application

• Determination of berberine in human serum by reversed-phase high performance liquid chromatography.: This study outlines a method for detecting berberine in human serum using reversed-phase HPLC, utilizing the LiChrosorb RP-18 column for its reliability in separating chemical compounds in biological samples (Sun Y et al., 1997).


• Kinetics of hydrolysis of 4-methoxy-2-[2-hydroxy-3(4-phenyl-1-piperazinyl)]propyl-2,3-dihydro-6-methyl-1,3-dioxo-1H-pyrrolo[3,4-c]pyridine in aqueous solutions.: This research employs RP-18 to study the stability and hydrolysis kinetics of a complex organic compound, highlighting its application in pharmaceutical analysis (Muszalska I et al., 2004).


• A validated spectrophotometric and liquid chromatography method for determination and purity evaluation of 4-methoxy-2-[2-hydroxy-3(4-phenyl-1-piperazinyl)]propyl-2,3-dihydro-6-methyl-1,3-dioxo-1H-pyrrolo[3,4-c]pyridine.: This paper details the use of RP-18 in validating a method for assessing the purity of pharmaceutical compounds, demonstrating its essential role in quality control (Muszalska I et al., 2003).


• High-performance liquid chromatographic determination of the antifungal drug fluconazole in plasma and saliva of human immunodeficiency virus-infected patients.: This study utilizes RP-18 to monitor fluconazole in clinical samples, providing insights into the pharmacokinetics and therapeutic monitoring of antifungal treatment in HIV patients (Beijnen JH et al., 1995).


• Ion-pair solid-phase extraction of cimetidine from plasma and subsequent analysis by high-performance liquid chromatography.: Describes a method using RP-18 for the extraction and analysis of cimetidine, a drug used to treat heartburn and peptic ulcers, from plasma samples, illustrating its versatility in drug analysis (Russel FG et al., 1994).

Analysis Note

Theoretical Plates (N/m) (Toluene): ≥ 60000
stability testing (performance): passes test
separation factor (Acetophenone/Phenol, selectivity test): 1.8 - 2.2
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.1 - 1.4
separation factor (1-Naphtol/Phenol, selectivity test): 3.9 - 4.7
capacity factor (Toluene): 16.0 - 21.0
Particle Size (d10): 3.8 - 4.3 µm
Particle Size (d50): 6.7 - 7.7 µm
Particle Size (d90): 12.0 - 14.0 µm

Legal Information

LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable