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1.09277

Sigma-Aldrich

Tuerk′s Solution

for leukocyte counting

Sinónimos:

Acetic acid gentian violet solution

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About This Item

UNSPSC Code:
41116121
NACRES:
NA.21
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

400

form

liquid

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

pH

2.5-3.5

density

1.00 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

Categorías relacionadas

General description

Tuerk′s solution - for leukocytes counting, is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the hematological investigation of sample material of human origin in hematological specimen materials. The solution serves the purpose of the manual semiquantitative determination of leukocytes in fresh venous, anticoagulated whole blood using a counting chamber.
The basis of all counting methods is the dilution and preparation of a blood sample of known volume. The erythrocytes are hemolyzed by the acetic acid present in Tuerk′s solution and the leukocyte nuclei are stained blue-violet by the dye. The required cell type in a defined volume is counted and the number of cells per microliter of blood is then calculated.
The 100ml bottle of Tuerks solution will provide approx. 10 applications. Tuerk′s solution is an IVD product and CE registered.For more details, please see instructions for use (IFU).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Questions

1–3 of 3 Questions  

  1. Can Tuerk's solution stain platelets/thrombocytes? Is there any other better stain for counting platelets?

    1 answer

    1. Tuerk's solution is not suitable for counting thrombocytes/platelets. It is advisable to use phase contrast microscopy for platelet counting, as Tuerk's solution may destroy platelets.

      Helpful?

  2. what is the concentration of Turks solution

    1 answer

    1. The concentration of the Turks solution is not determined.

      Helpful?

  3. Can I count PBMC with Turk Solution? Or what are the best option for PBMC counting? and another question Can Turk stain Thrombocytes?

    1 answer

    1. Tuerk's Solution is primarily used for counting the total white cells present in a peripheral blood specimen, including PBMCs (lymphocytes and monocytes).
      For accurate counting of PBMCs, the most common approach is to use flow cytometry or hemocytometers. Hematology labs will typically have dedicated cell counters that will also count white blood cells and also classify the percentage of each type of white blood cell found in the blood specimen. Alternatively, instruments, such as the Scepter™ cell counter, will differentiate PBMCs based on size.

      See the link below to review an application note that may be of interest:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/775/an3311en-ms.pdf

      Tuerk's solution is not routinely used for counting thrombocytes or platelets. Although some platelets may show some staining, there will be platelets that remain unstained. Most labs that count platelets manually will use ammonium oxalate to lyse the red blood cells and then use a phase hemacytometer and a phase (contrast) microscope.

      Helpful?

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