07-820

Anti-phospho-EGFR (Tyr845) Antibody

Upstate^®, from rabbit

• Sinónimos: Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NM_005228.3; NM_201282.1; NM_201283.1; NM_201284.1
• UniProt accession no.: P00533
• shipped in: dry ice
• target post-translational modification: phosphorylation (pTyr845)
• Gene Information: human ... EGFR(1956)

Descripción

Specificity

Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.

Recognizes phospho-EGFR (Tyr845).

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr845)

Application

Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr845) in RIPA lysates from EGF stimulated A431 cells.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-phospho-EGFR (Tyr845) Antibody is validated for use in IC, IF, IH, WB for the detection of phospho-EGFR (Tyr845).

Quality

Routinely evaluated by immunoblot.

Target description

185 kDa

Physical form

Affinity Purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

ImmunoAffinity Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
EGF stimulated A431 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable