07-215
Anti-dimethyl-Histone H3 (Arg26) Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
H3R26me2, Histone H3 (di methyl R26), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
vertebrates, Tetrahymena sp., Xenopus, human
technique(s)
multiplexing: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
dimethylation (Arg26)
Gene Information
human ... H3C1(8350)
General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Specificity
Recognizes CARM1 in vitro methylated recombinant Histone H3. The immunizing sequence is conserved from Tetrahymena through human therefore a broad cross reactivity is expected.
Recognizes dimethylated Histone H3 at and around arginine 26, MW ~17 kDa. Possible slight reactivity with monomethyl histone H3 (Arg26).
Immunogen
Epitope: Dimethylated Histone H3 (Arg26)
KLH-conjugated synthetic peptide (ATKAA(di-Me)RKSAPA-C) corresponding to amino acids 21-31 of Human Histone H3.
Application
Anti-dimethyl-Histone H3 (Arg26) Antibody is a rabbit polyclonal antibody for detection of dimethyl-Histone H3 (Arg26) also known as H3R26me2, Histone H3 (di methyl R26) & has been validated in WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
SNAP I.D. (Western Blotting Technique):
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.
Quality
Evaluated by western blot on untreated and CARM1 in vitro methylated recombinant Histone H3.
Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.
Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.
Target description
17kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl, with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
CARM1 in vitro methylated recombinant Histone H3.
CARM1 in vitro methylated recombinant Histone H3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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El Messaoudi, S; Fabbrizio, E; Rodriguez, C; Chuchana, P; Fauquier, L; Cheng, D; Theillet et al.
Proceedings of the National Academy of Sciences of the USA null
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