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Key Documents

07-130

Sigma-Aldrich

Anti-Ezrin Antibody

Upstate®, from rabbit

Sinónimos:

Anti-CVIL, Anti-CVL, Anti-HEL-S-105, Anti-VIL2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Ezr(22350)

General description

Ezrin is also referred to as Villin-2 and Cytovillin

Specificity

Ezrin

Immunogen

peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG)

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Ezrin Antibody is validated for use in WB, IH for the detection of Ezrin.

Quality

routinely evaluated by immunoblot on RIPA lysates from A431 cells or a mouse brain lysate

Target description

81kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Ana Cicvaric et al.
Annals of medicine, 48(8), 652-668 (2016-08-26)
Podoplanin is a cell-surface glycoprotein constitutively expressed in the brain and implicated in human brain tumorigenesis. The intrinsic function of podoplanin in brain neurons remains however uncharacterized. Using an established podoplanin-knockout mouse model and electrophysiological, biochemical, and behavioral approaches, we
Proteomics analysis of the ezrin interactome in B cells reveals a novel association with Myo18a?.
Ken Matsui,Neetha Parameswaran,Nayer Bagheri,Belinda Willard,Neetu Gupta
Journal of Proteome Research null
Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement
Potter, D. A., et al
The Journal of Biological Chemistry, 278, 30403-30412 (2003)
Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins.
S Tsukita et al.
The Journal of biological chemistry, 274(49), 34507-34510 (1999-11-27)
Teresita N J Flores-Téllez et al.
PloS one, 10(7), e0131605-e0131605 (2015-07-03)
Prognostic markers are important for predicting the progression and staging of hepatocellular carcinoma (HCC). Ezrin (EZR) and Podocalyxin (PODXL) are proteins associated with invasion, migration and poor prognosis in various types of cancer. Recently, it has been observed that chloride

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