05-1078

Anti-STAT3 Antibody, clone 18F7.1

clone 18F7.1, from mouse

• Sinónimos: Acute-phase response factor ; ; DNA-binding protein APRF ; ; signal transducer and activator of transcription 3 ; ; signal transducer and activator of transcription 3 ; ; (acute-phase response factor)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 18F7.1, monoclonal
• reactividad de especies: mouse, human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_003141
• Nº de acceso UniProt: P40763
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... STAT3(6774) ; mouse ... Stat3(20848)

Descripción

Descripción general

STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.

Inmunógeno

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human STAT3.

Aplicación

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STAT3 in HeLa and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-STAT3 Antibody, clone 18F7.1 (mouse monoclonal antibody) validated in WB, ICC to detect STAT3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.

Calidad

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 in 10 µg of HeLa cell lysate.

Descripción de destino

~88 kDa observed

Ligadura / enlace

Replaces: 04-1014

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable