04-784
Anti-IRS1 Antibody, clone AW58, rabbit monoclonal
culture supernatant, clone AW58, Upstate®
Sinónimos:
insulin receptor substrate 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
AW58, monoclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... IRS1(3667)
General description
Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.
Specificity
IRS1
Immunogen
Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1
Application
Anti-IRS1 Antibody, clone AW58 detects level of IRS1 & has been published & validated for use in IP & WB.
Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.
Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.
Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Quality
routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts
Target description
Mr ~145-165kDa
Linkage
Replaces: 05-784
Physical form
Cultured supernantant in 0.05% sodium azide
Storage and Stability
1 year at -20°C from date of shipment
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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