There is very little difference between these two products, based on physical properties. Each product originated from a different brand with the R7017 originating from the Sigma brand while the 199303 was originally an Aldrich brand. In addition, the R7017 has a dye content of ~80% and is listed as a BioReagent, meaning the tests performed are more relevant to molecular biology or cell culture applications. Product 199303 has 75% dye content and is certified by the Biological Stain Commission. These Certified dyes are tested to ensure the quality of dyes through independent testing to meet appropriate chemical and performance criteria. In most application areas, these two products would be interchangeable.
199303
Resazurin sodium salt
certified by the Biological Stain Commission
Sinónimos:
7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
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About This Item
Fórmula empírica (notación de Hill):
C12H6NNaO4
Número de CAS:
Peso molecular:
251.17
Beilstein/REAXYS Number:
3794971
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Productos recomendados
grade
certified by the Biological Stain Commission
Quality Level
form
powder
composition
Dye content, 75%
solubility
1 M NH4OH: 1 mg/mL
λmax
380 nm (2nd)
598 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
InChI
1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
InChI key
IVGPGQSSDLDOLH-UHFFFAOYSA-M
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General description
Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.[1]
Application
For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:
- the measurement of metabolic activity and the proliferation of living cells
- the detection of bacterial reduction in milk
- in viability assays as a component of culture media for the detection of viable bacteria
- to determine the number of motile sperm in sheep semen
- to assay lactate dehydrogenase
- as an analytical reagent to quantify L-glutamate[1]
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Can you tell me the precise difference between R7017 and 199303? I am interested in exposing live cells to Resazurin salt. Is one of these two products more appropriate for that purpose?
1 answer-
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