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SRP3114

Sigma-Aldrich

Midkine human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

MK, NEGF-2

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

13.4 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MDK(4192)

General description

Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.

Application

Midkine human has been used in cell viability assay and cell proliferation assay to determine its association with EMT (epithelial-mesenchymal transition) and gemcitabine resistance in biliary tract cancer.

Biochem/physiol Actions

Midkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK (midkine) plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages. Signaling through the ALK (anaplastic lymphoma kinase) receptor MK exerts angiogenic, growth and survival activities during tumorigenesis. MK is expressed predominantly during midgestation in the brain and several other organs and is down-regulated at birth.

Sequence

VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Physical form

Lyophilized from 0.4x PBS, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Therapeutic potential of midkine in cardiovascular disease.
Kadomatsu K, et al.
British Journal of Pharmacology, 171(4), 936-944 (2014)
Effect of midkine on gemcitabine resistance in biliary tract cancer.
Lu Y, et al.
International Journal of Molecular Medicine, 41(4), 2003-2011 (2018)
Hui-Lian Huang et al.
International journal of clinical and experimental pathology, 8(5), 5387-5394 (2015-07-21)
To investigate the inhibitory effect of midkine-binding peptides on human umbilical vein endothelial cells (HUVECs) proliferation and angiogenesis of xenograft tumor. The midkine-binding peptides were panned by Ph.D.-7(™) Phage Display Peptide Library Kit, and the specific binding activities of positive
Midkine regulates pleiotrophin organ-specific gene expression: evidence for transcriptional regulation and functional redundancy within the pleiotrophin/midkine developmental gene family.
Herradon G, et al.
Biochemical and Biophysical Research Communications, 333(3), 714-721 (2005)
The growth factor midkine is modulated by both glucocorticoid and retinoid in fetal lung development.
Kaplan F, et al.
American Journal of Respiratory Cell and Molecular Biology, 28(1), 33-41 (2003)

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