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SAB4200818

Sigma-Aldrich

Anti- Proteus mirabilis antibody produced in rabbit

IgG fraction of antiserum

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

antibody form

IgG fraction of antiserum

clone

polyclonal

description

Research area: Microbiome

form

buffered aqueous solution

mol wt

~70 kDa

species reactivity

Proteus mirabilis

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 1:10,000-1:20,000 using Proteus mirabilis LPS
indirect ELISA: 1:16,000-1:32,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Proteus mirabilis is a Gram negative rod-shaped bacteria, belongs to the Enterobacteriaceae family. Member of the Proteus genus (Proteus spp.) which also includes Proteus mirabilis, Proteus penneri and Proteus hauseri, originally characterize by their ability to swarm on solid surfaces, are widespread in the environment and the gastrointestinal tract of human and animals and known to be an opportunistic pathogens isolated from urine, wounds and other clinical sources. The Proteus spp. bacteria, are distinguished by their reactions for indole production, salicin fermentation and aesculin hydrolysis. P. vulgaris produces indole which differentiates it from the indole-negative P. mirabilis and P. penneri. Proteus spp. bacteria may also be found in soil or water habitats where they often regarded as indicators of fecal pollution and a contamination threat for potential water or seafood poisoning.

Immunogen

Proteus mirabilis OXK dead bacteria, ATCC strain 15146

Application

Anti-Proteus mirabilis antibody recognizes P. mirabilis whole extract and P. mirabilis LPS, the antibody also recognizes an additional ~70kDa band suspected as bacterial HSP70 (DNAK) in whole extract P. vulgaris, P. gingivalis, E.coli K-12, P.aeruginosa, S. flexneri, S. enterica and E. faecalis but it has no cross reactivity with P. vulgaris LPS. The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA. 

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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C M O'Hara et al.
International journal of systematic and evolutionary microbiology, 50 Pt 5, 1869-1875 (2000-10-18)
Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as Proteus penneri. Biogroup 2, characterized by positive reactions for indole, salicin and aesculin
ANTIMICROBIAL RESISTANCE PATTERNS OF PROTEUS ISOLATES FROM CLINICAL SPECIMENS
Bahashwan, et al.
EUROPEAN SCIENTIFIC JOURNAL, 9, 188-202 (2013)
Noriyuki Nagano et al.
Journal of clinical microbiology, 41(12), 5530-5536 (2003-12-10)
Nineteen multidrug-resistant Proteus mirabilis strains were isolated from 19 patients suffering from infections probably caused by P. mirabilis. These strains were recovered from urine or other urogenital specimens of 16 inpatients and three outpatients with a hospitalization history in a
Jessica N Schaffer et al.
Microbiology spectrum, 3(5) (2015-11-07)
Proteus mirabilis is a Gram-negative bacterium and is well known for its ability to robustly swarm across surfaces in a striking bulls'-eye pattern. Clinically, this organism is most frequently a pathogen of the urinary tract, particularly in patients undergoing long-term
Dominika Drzewiecka
Microbial ecology, 72(4), 741-758 (2016-10-27)
Proteus spp. bacteria were first described in 1885 by Gustav Hauser, who had revealed their feature of intensive swarming growth. Currently, the genus is divided into Proteus mirabilis, Proteus vulgaris, Proteus penneri, Proteus hauseri, and three unnamed genomospecies 4, 5

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