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Key Documents

R6626

Sigma-Aldrich

Rhodamine B

≥95% purity (HPLC), powder

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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About This Item

Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
Molecular Weight:
479.01
Colour Index Number:
45170
Beilstein:
4091619
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Rhodamine B, ≥95% (HPLC)

Assay

≥95% (HPLC)

form

powder

color

faint green to very dark green

mp

210-211 (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

0.79 g/cm3

ε (extinction coefficient)

104500-115800 at 542-554 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

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General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation. It is used as a biomarker in oral rabies vaccine.

Application

Rhodamine B has been used:
  • to represent fluorescence of tetra methyl rhodamine (TMR)
  • in the preparation of adhesive resin for confocal laser scanning microscopy
  • as a fluorescent dye in intrahemolymph dye dosing and retinal image acquisition
  • to depict brain-specific dye capture
  • for imaging targets

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Pictograms

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Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Micromorphology of resin/dentin interfaces using 4th and 5th generation dual-curing adhesive/cement systems: a confocal laser scanning microscope analysis
Arrais CAG, et al.
The Journal of Adhesive Dentistry (2009)
Fine depth resolution of two-photon absorption-induced photoacoustic microscopy using low-frequency bandpass filtering
YamaokaY, et al.
Optics Express, 19(14), 13365-13377 (2011)
John F. Breidenstein
A study of water circulation in Monterey harbor using rhodamine B dye (1965)
Palmitate mediated diacylglycerol accumulation causes endoplasmic reticulum stress, Plin2 degradation, and cell death in H9C2 cardiomyoblasts
AliAkoumi
Experimental Cell Research (2017)
Novel models for studying the blood-brain and blood-eye barriers in Drosophila
Nag S, et al.
The Blood-Brain and Other Neural Barriers, 357-369 (2011)

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