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H0527

Sigma-Aldrich

HEPES potassium salt

≥99.5% (titration)

Synonym(s):

Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-monopotassium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17KN2O4S
CAS Number:
82207-62-3
Molecular Weight:
276.39
MDL number:
MFCD00043438
UNSPSC Code:
12161700
PubChem Substance ID:
24895405
NACRES:
NA.25

Quality Level

200

Assay

≥99.5% (titration)

form

powder

useful pH range

6.8-8.2

pKa 

7.5

solubility

water: 0.5 g/mL, clear, colorless

SMILES string

[K+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.K/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

KWURIFKZTJGMFD-UHFFFAOYSA-M

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General description

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a widely used Good’s buffer in biochemical and biological research applications. As a zwitterionic buffer, it stabilizes the pH of the solution even when small amounts of acid or base are added.

Application

HEPES potassium salt has been used as a buffering agent in nanopore fabrication and electrolyte preparations. It has also been used as a component of cell lysis buffer.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HEPES

Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction
Chu CC and Li HM
Methods in Molecular Biology (2011)
Complexation of copper by zwitterionic aminosulfonic (good) buffers
Mash HE, et al.
Analytical Chemistry (2003)
Use of a solid-state nanopore for profiling the transferrin receptor protein and distinguishing between transferrin receptor and its ligand protein
O'Donohue M, et al.
Electrophoresis (2023)
Equilibrium studies, synthesis and characterisation of zwitterionic buffer (HEPES and HEPPS) dimethyltin (IV) complexes
Mohamed M
Main Group Chemistry, 63-84 (2007)
Shankar Dutt et al.
Analytical chemistry, 95(13), 5754-5763 (2023-03-18)
Thin membranes are highly sought-after for nanopore-based single-molecule sensing, and fabrication of such membranes becomes challenging in the ≲10 nm thickness regime where a plethora of useful molecule information can be acquired by nanopore sensing. In this work, we present
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