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Key Documents

C7642

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

FITC conjugate, Type IV, lyophilized powder

Synonym(s):

ConA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Canavalia ensiformis

conjugate

FITC conjugate

type

Type IV

form

lyophilized powder

composition

Protein, 5-15% biuret

extent of labeling

3-6 mol FITC per mol protein

impurities

Carbohydrate, essentially free

storage temp.

−20°C

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Application

For the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing phosphate buffer salts and NaCl

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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