ABE1306

Anti-DOM3Z Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Decapping and exoribonuclease protein, DXO, Dom-3 homolog Z, DOM3Z
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat, human
• species reactivity (predicted by homology): bovine (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_005501
• UniProt accession no.: O77932
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DXO(1797)

Description

General description

Decapping and exoribonuclease protein (DOM3Z), also known as DXO, Dom-3 homolog Z, and encoded by the gene symbol DOM3Z/ DOM3L/ NG6, is a ribonuclease that specifically degrades pre-mRNAs with a defective 5′ end cap. DOM3Z is part of a pre-mRNA capping quality control and has decapping, pyrophosphohydrolase and 5′-3′ exonuclease activities. DOM3Z has a decapping activity toward incomplete 5′ end cap mRNAs (such as unmethylated 5′ end-capped RNA), which in return releases GpppN and 5′ end monophosphate RNA. The 5′ end monophosphate RNA is then degraded by the 5′-3′ exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Additionally, DOM3Z has been implied to have RNA 5′-pyrophosphohydrolase activity and to release pyrophosphates by hydrolyzing the 5′ end triphosphate. DOM3Z is located in the nucleus, and expressed ubiquitously.

Immunogen

KLH-conjugated linear peptide corresponding to human DOM3Z.

Application

Anti-DOM3Z is an antibody against DOM3Z for use in Western Blotting.

Quality

Evaluated by Western Blotting in human pancreas tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DOM3Z in 10 µg of human pancreas tissue lysate.

Target description

~45 kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable