189725
Avidin, Egg White
Synonym(s):
Avidin, Egg White
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About This Item
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form
lyophilized solid
Quality Level
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
dilute buffer: 5 mg/mL
distilled water: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
Avidin is a tetrameric glycoprotein and an anti-nutrient found in the eggs of all oviparous vertebrates. It constitutes 0.05–0.5% of the total protein in hen eggs. Avidin structure contains eight orthogonal antiparallel β-strands, a single disulfide bond, and a carbohydrate side chain.
Application
Avidin, Egg White has been used as a blocking agent in protein-binding assays. It has also been used to form avidin-biotin linkage to conjugate gemcitabine onto microbubbles for drug delivery to in vivo model.
Biochem/physiol Actions
Avidin can be used as a label for antibodies, fluorescent dyes, proteins,, and other molecules of interest to biochemists. The high binding affinity of avidin to biotin (vitamin H) causes the unavailability of biotin to microorganisms, which leads to the inhibition of their proliferation. This feature of avidin contributes to its antimicrobial property and affects biotin absorption in the body, resulting in vitamin B7 deficiency.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of avidin that will bind 1 µg D-biotin at pH 8.9.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C. Avoid freeze/thaw cycles.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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